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- Title
Identification of a vaccine candidate antigen, PfMAg-1, from Plasmodium falciparum with monoclonal antibody M26-32.
- Authors
Gao, Yu-Hui; Li, Hui-Liang; Lu, Yan; Gao, Fang-Ming; Lin, Ya-Hui; Zhou, Hong-Chang; Zhang, Lian-Hui; Wang, Heng
- Abstract
Abstract  Monoclonal antibody M26-32 has been shown to strongly inhibit the growth of Plasmodium falciparum in vitro. To identify the target antigen of M26-32, a P. falciparum Dd2 asexual stage cDNA expression library was screened with this antibody, and a full open reading frame cDNA was obtained. This gene, named pfmag-1, encodes a polypeptide of 589 amino acids. The protein PfMAg-1 was characterized as a membrane-associated protein that expressed on the surface of merozoite during erythrocytic stage. Remarkably, at the C terminus of PfMAg-1, there are 14 copies of a deca-peptide sequence of QTDEIKND (H/N) I. This tandem repeat domain was identified to harbor the epitope of the protective M26-32 monoclonal antibody, and was also recognized by sera of patients infected with P. falciparum. Rabbit antibody elicited against this deca-peptide repeat domain effectively inhibited P. falciparum invasion in vitro. Our work suggests that PfMAg-1 is a promising malaria vaccine candidate.
- Subjects
PARASITE antigens; PLASMODIUM falciparum; VACCINES; MONOCLONAL antibodies; GENE expression; COMPLEMENTARY DNA
- Publication
Parasitology Research, 2009, Vol 105, Issue 6, p1723
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-009-1617-4