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- Title
Adhesion molecule–targeted magnetic particle imaging nanoprobe for visualization of inflammation in acute lung injury.
- Authors
Gao, Pengli; Liu, Yu; Wang, Xiaoli; Feng, Xin; Liu, Heng; Liu, Songlu; Huang, Xiazi; Wu, Xiangjun; Xiong, Fei; Jia, Xiaohua; Hui, Hui; Jiang, Jingying; Tian, Jie
- Abstract
Purpose: Uncontrolled intra-alveolar inflammation is a central pathogenic feature, and its severity translates into a valid prognostic indicator of acute lung injury (ALI). Unfortunately, current clinical imaging approaches are unsuitable for visualizing and quantifying intra-alveolar inflammation. This study aimed to construct a small-sized vascular cell adhesion molecule-1 (VCAM-1)-targeted magnetic particle imaging (MPI) nanoprobe (ESPVPN) to visualize and accurately quantify intra-alveolar inflammation at the molecular level. Methods: ESPVPN was engineered by conjugating a peptide (VHPKQHRGGSK(Cy7)GC) onto a polydopamine-functionalized superparamagnetic iron oxide core. The MPI performance, targeting, and biosafety of the ESPVPN were characterized. VCAM-1 expression in HUVECs and mouse models was evaluated by western blot. The degree of inflammation and distribution of VCAM-1 in the lungs were assessed using histopathology. The expression of pro-inflammatory markers and VCAM-1 in lung tissue lysates was measured using ELISA. After intravenous administration of ESPVPN, MPI and CT imaging were used to analyze the distribution of ESPVPN in the lungs of the LPS-induced ALI models. Results: The small-sized (~10 nm) ESPVPN exhibited superior MPI performance compared to commercial MagImaging® and Vivotrax, and ESPVPN had effective targeting and biosafety. VCAM-1 was highly expressed in LPS-induced ALI mice. VCAM-1 expression was positively correlated with the LPS-induced dose (R = 0.9381). The in vivo MPI signal showed positive correlations with both VCAM-1 expression (R = 0.9186) and representative pro-inflammatory markers (MPO, TNF-α, IL-6, IL-8, and IL-1β, R > 0.7). Conclusion: ESPVPN effectively targeted inflammatory lungs and combined the advantages of MPI quantitative imaging to visualize and evaluate the degree of ALI inflammation.
- Subjects
MAGNETIC particle imaging; LUNGS; VASCULAR cell adhesion molecule-1; LUNG injuries; LIPOPOLYSACCHARIDES; PNEUMONIA; DATA visualization
- Publication
European Journal of Nuclear Medicine & Molecular Imaging, 2024, Vol 51, Issue 5, p1233
- ISSN
1619-7070
- Publication type
Article
- DOI
10.1007/s00259-023-06550-4