We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of a novel quaternary ammonium silane cavity disinfectant on cariogenic biofilm formation.
- Authors
Daood, U.; Burrow, M. F.; Yiu, C. K. Y.
- Abstract
Objective: Evaluate effect of quaternary ammonium silane (QAS) cavity disinfectant on cariogenic biofilm. Materials and methods: Single- (Streptococcus mutans or Lactobacillus acidophilus), dual- (Streptococcus mutans/Lactobacillus Acidophilus), and multi-species (Streptococcus mutans, Actinomyces naeslundii, and Streptococcus sanguis) biofilms were grown on acid-etched dentine discs. Biofilms were incubated (120 min/37 °C) and allowed to grow for 3 days anaerobically. Discs (no treatment) served as control (group 1). Groups II, III, IV, and V were then treated with 2% chlorhexidine, and 2%, 5%, and 10% QAS (20 s). Discs were returned to well plates with 300 μL of bacterial suspension and placed in anaerobic incubator at 37 °C and biofilms redeveloped for 4 days. Confocal microscopy, Raman, CFU, and MTT assay were performed. Results: Raman peaks show shifts at 1450 cm−1, 1453 cm−1, 1457 cm−1, 1460 cm−1, and 1462 cm−1 for control, 2% CHX, 2%, 5%, and 10% QAS groups in multi-species biofilms. There was reduction of 484 cm−1 band in 10% QAS group. CLSM revealed densely clustered green colonies in control group and red confluent QAS-treated biofilms with significantly lower log CFU for single/dual species. Metabolic activities of Streptococcus mutans and Lactobacillus acidophilus decreased with increasing QAS exposure time. Conclusion: Quaternary ammonium silanes possess antimicrobial activities and inhibit growth of cariogenic biofilms. Clinical significance: Available data demonstrated use of QAS as potential antibacterial cavity disinfectant in adhesive dentistry. Experimental QAS can effectively eliminate caries-forming bacteria, when used inside a prepared cavity, and can definitely overcome problems associated with present available cavity disinfectants.
- Subjects
CARIOGENIC agents; DISINFECTION &; disinfectants; LACTOBACILLUS acidophilus; STREPTOCOCCUS mutans; STREPTOCOCCUS sanguis; DENTAL adhesives
- Publication
Clinical Oral Investigations, 2020, Vol 24, Issue 2, p649
- ISSN
1432-6981
- Publication type
Article
- DOI
10.1007/s00784-019-02928-7