We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Aging of human epidermis: reversal of aging changes correlates with reversal of keratinocyte fas expression and apoptosis.
- Authors
Gilhar, Amos; Ullmann, Yehuda; Karry, Rachel; Shalaginov, Raya; Assy, Bedia; Serafimovich, Sima; Kalish, Richard S.; Smith, James R.
- Abstract
The goal of this study was to determine the role of Fas-mediated apoptosis in human epidermal aging. Epidermal Fas expression and apoptosis are increased in aged human skin. Aging changes of human epidermis, including decreased epidermal thickness and proliferation, are reversed following grafting of human skin to SCID (severe combined immunodeficiency) mice. Skin from aged participants (n = 14; mean 70.7 years), and young participants (n = 14; mean 23.4 years) was grafted to beige SCID mice, and epidermal thickness, proliferation (Ki-67 expression), apoptosis (TUNEL [Tdt-mediated dUTP nick end labeling] reaction below granular layer), and expression of Fas and FasL were determined by histology and immunochemical staining. Aged skin was associated with thinning of the epidermis, decreased epidermal proliferation, a significant increase in apoptosis below the granular layer, and epidermal Fas expression. Engraftment significantly reversed these aging changes, including apoptosis, and Fas expression. Correlation of reversal of aging changes, with decreased epidermal Fas expression and apoptosis, supports a role for Fas-mediated apoptosis in aging of human epidermis.
- Subjects
AGING; SKIN; EPIDERMIS; APOPTOSIS; CELL death; HISTOLOGY
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2004, Vol 59, Issue 5, p411
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/59.5.B411