We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Duplex Real-Time PCR Assays for the Simultaneous Detection and Quantification of Botryosphaeriaceae Species Causing Canker Diseases in Woody Crops.
- Authors
Romero-Cuadrado, Laura; López-Herrera, Carlos José; Aguado, Ana; Capote, Nieves
- Abstract
Woody canker diseases caused by fungi of the Botryosphaeriaceae family are producing increasing losses in many economically important woody crops, including almond. To develop a molecular tool for the detection and quantification of the most aggressive and threatening species is of main importance. This will help to prevent the introduction of these pathogens in new orchards and to conveniently apply the appropriate control measures. Three reliable, sensitive and specific duplex qPCR assays using TaqMan probes have been designed for the detection and quantification of (a) Neofusicoccum parvum and the Neofusicoccum genus, (b) N. parvum and the Botryosphaeriaceae family and (c) Botryosphaeria dothidea and the Botryosphaeriaceae family. The multiplex qPCR protocols have been validated on artificially and naturally infected plants. Direct systems to process plant materials, without DNA purification, allowed high-throughput detection of Botryosphaeriaceae targets even in asymptomatic tissues. These results validate the qPCR using the direct sample preparation method as a valuable tool for Botryosphaeria dieback diagnosis allowing a large-scale analysis and the preventive detection of latent infection.
- Subjects
BOTRYOSPHAERIACEAE; CANKER (Plant disease); PLANT diseases; LATENT infection; SPECIES; MYCOSES
- Publication
Plants (2223-7747), 2023, Vol 12, Issue 11, p2205
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants12112205