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- Title
The function of PML in p53-dependent apoptosis.
- Authors
Guo, Ailan; Salomoni, Paolo; Luo, Jianyuan; Shih, Alan; Zhong, Sue; Gu, Wei; Paolo Pandolfi, Pier
- Abstract
The PML gene of acute promyelocytic leukaemia (APL) encodes a growth- and tumour-suppresor protein that is essential for several apoptotic signals. The mechanisms by which PML exerts its pro-apoptotic function are still unknown. Here we show that PML acts as a transcriptional co-activator with p53. PML physically interacts with p53 bothin vitro andin vivo and co-localizes with p53 in the PML nuclear body (PML-NB). The co-activatory role of PML depends on its ability to localize in the PML-NB. p53-dependent, DNA-damage-induced apoptosis, transcriptional activation by p53, the DNA-binding ability of p53, and the induction of p53 target genes such asBax andp21 upon γ-irradiation are all impaired inPML(-/-)primary cells. These results define a new PML-dependent, p53-regulatory pathway for apoptosis and shed new light on the function of PML in tumour suppression.
- Subjects
LEUKEMIA; P53 protein; GENES; TUMORS
- Publication
Nature Cell Biology, 2000, Vol 2, Issue 10, p730
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/35036365