We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Mast cells play a key role in the developmentof late airway hyperresponsiveness through TNF-αin a murine model of asthma.
- Authors
Kim, Young-Suk; Ko, Hyun-Mi; Kang, Nam-In; Song, Chang-Ho; Zhang, Xin; Chung, Woo-Chan; Kim, Jae-Hong; Choi, In-Hak; Park, Young-Min; Kim, Gi-Young; Im, Shun-Young; Lee, Hern-Ku
- Abstract
We have investigated the role of TNF-α in mast cell-mediated late airway hyperresponsiveness (AHR) using mast cell-deficient WBB6F1- W/W ( W/W) mice in a murine model of asthma, which exhibits a biphasic increase in AHR. TNF-α levels in the airway and magnitude of late AHR in response to airway allergen challenge were severely impaired in W/W mice compared to their littermates. In addition to TNF-α, cytosolic phospholipase A (cPLA) phosphorylation and enzymatic activity in the lungs were also impaired in W/W mice. Either anti-TNF-α antibody or an inhibitor of cPLA abolished late AHR in congeneic +/+ mice. Intratracheal administration of TNF-α resulted in increases in late AHR, cPLAphosphorylation, cPLAactivity, and phosphorylation of mitogen-activated protein kinases. Mast cell replacement restored airway TNF-α level, cPLAphosphorylation and enzymatic activity in the lungs as well as late AHR in W/W mice. These data indicate that mast cells play a key role in the development of late AHR through liberation of TNF-α.
- Publication
European Journal of Immunology, 2007, Vol 37, Issue 4, p1107
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200636612