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- Title
ZNF143 mediates CTCF-bound promoter–enhancer loops required for murine hematopoietic stem and progenitor cell function.
- Authors
Zhou, Qiling; Yu, Miao; Tirado-Magallanes, Roberto; Li, Bin; Kong, Lingshi; Guo, Mingrui; Tan, Zi Hui; Lee, Sanghoon; Chai, Li; Numata, Akihiko; Benoukraf, Touati; Fullwood, Melissa Jane; Osato, Motomi; Ren, Bing; Tenen, Daniel G.
- Abstract
CCCTC binding factor (CTCF) is an important factor in the maintenance of chromatin–chromatin interactions, yet the mechanism regulating its binding to chromatin is unknown. We demonstrate that zinc finger protein 143 (ZNF143) is a key regulator for CTCF-bound promoter–enhancer loops. In the murine genome, a large percentage of CTCF and ZNF143 DNA binding motifs are distributed 37 bp apart in the convergent orientation. Furthermore, deletion of ZNF143 leads to loss of CTCF binding on promoter and enhancer regions associated with gene expression changes. CTCF-bound promoter–enhancer loops are also disrupted after excision of ZNF143. ZNF143-CTCF-bound promoter–enhancer loops regulate gene expression patterns essential for maintenance of murine hematopoietic stem and progenitor cell integrity. Our data suggest a common feature of gene regulation is that ZNF143 is a critical factor for CTCF-bound promoter–enhancer loops. CTCF mediates DNA–DNA interactions by forming dimers that directly bind to both sides of the DNA. Here the authors reveal that a CTCF known associated zinc finger, ZNF143, mediates gene transcription through CTCF–DNA binding to maintain murine hematopoietic stem and progenitor cell integrity.
- Subjects
CELL physiology; ZINC-finger proteins; GENETIC regulation; PROMOTERS (Genetics); GENE expression; HEMATOPOIETIC stem cells
- Publication
Nature Communications, 2021, Vol 12, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-020-20282-1