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- Title
Effects of upstream deletions on light- and oxygen-regulated bacterio-opsin gene expression in Halobacterium halobium.
- Authors
Gropp, Felix; Gropp, Roswitha; Betlach, Mary C.
- Abstract
The bacterio-opsin gene (bop) of <em>Halobacterium halobium</em> is located within a cluster with three other genes. Growth conditions of high light intensity and low oxygen tension induce <em>bop</em> gene cluster expression. To identify putative regulatory factor binding sites upstream of the <em>bop</em> gene, we have compared sequences upstream of the <em>bop</em> gene with the corresponding sequences from two other genes in the <em>bop</em> gene cluster. Conserved sequence motifs were observed which may mediate the effect of high light intensity and/or low oxygen tension on <em>bop</em> gene expression. Based on these motifs, a set of mutants was constructed which contained deletions upstream of the <em>bop</em> gene. These constructs were tested in a host strain where <em>bop</em> gene expression is independent of oxygen regulation and in another strain where it is regulated by oxygen and light. The minimal upstream sequence required for both light- and oxygen-regulated <em>bop</em> gene expression was determined to be 54 bp.
- Subjects
GENE expression; GENETIC regulation; HALOBACTERIUM salinarium; GENES; BINDING sites
- Publication
Molecular Microbiology, 1995, Vol 16, Issue 2, p357
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1995.tb02307.x