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- Title
Effects of basic fibroblast growth factor on human periodontal ligament cells.
- Authors
Takayama, S.; Murakami, S.; Miki, Y.; Ikezawa, K.; Tasaka, S.; Terashima, A.; Asano, T.; Okada, H.
- Abstract
In order to clarify the regulatory mechanisms of periodontal regeneration by basic fibroblast growth factor (bFGF), effects of bFGF on proliferation, alkaline phosphatase activity, calcified nodule formation and extracellular matrix synthesis of human periodontal ligament (PDL) cells were examined in this study, bFGF enhanced the proliferative responses of PDL cells in a dose-dependent manner. The maximum mitogenic effect of bFGF on PDL cells was observed at the concentration of 10 ng/ml. Incontrast, bFGF inhibited the induction of alkaline phosphatase activity and the mineralized nodule formation by PDL cells. Moreover, employing the reverse transcription-polymerase chain reaction (RT-PCR) technique, we observed that the levels of laminin mRNA of human PDL cells was specifically upregulated by bFGF stimulation, but that of type I collagen mRNA was downregulated. On the other hand, the expression of type III collagen and fibronect n mRNA were not altered even when the cells were activated by bFGF. These result suggest that suppressing cytodiffereniation of PDL cells into mineralized tissue forming cells, bFGF may paly a role in would healing by inducing growth of immature PDL cells that in turn accelerates periodontal regeneration.
- Subjects
COLLAGEN; MESSENGER RNA; EXTRACELLULAR matrix; CALCIFICATION; BIOMINERALIZATION; POLYMERASE chain reaction
- Publication
Journal of Periodontal Research, 1997, Vol 32, Issue 8, p667
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.1997.tb00577.x