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- Title
Long-term effects of idiotype vaccination on the specific T-cell response in peripheral blood and bone marrow of multiple myeloma patients.
- Authors
Abdalla, Amir Osman; Hansson, Lotta; Eriksson,3, Ingrid; Näsman-Glaser, Barbro; Mellstedt, Håkan; Österborg, Anders
- Abstract
Objectives: To elucidate long-term effects of idiotype (Id) vaccination on Id-specific T cells of multiple myeloma (MM) patients and compare Id-specific T-cell responses of peripheral blood with those of bone marrow (BM). Materials and methods: Id-specific T-cell responses of peripheral blood mononuclear cells (PBMC) were compared with those of BM mononuclear cells (BMMC) in 10 MM patients vaccinated with the Id protein at a median time of 41 months since the last immunization. The PBMC responses at late follow-up were also compared with those during active immunization. The responses were assessed by a proliferation assay, enzyme-linked immunospot (ELISPOT) (γ-interferon), cytometric bead array (CBA) for secreted cytokines and quantitative real-time polymerase chain reaction (QRT-PCR) for cytokine gene expression. Results: At the late testing time, an Id-specific response was detected in PBMC of five patients (ELISPOT, CBA, QRT-PCR) and in BMMC of four patients (CBA, QRT-PCR). A response in both compartments was noted only in three patients. The cytokines gene profile was consistent with a predominance of Th2 cells [interleukin (IL)-4, IL-5, IL-10]. Comparison of the Id-specific responses of PBMC during active immunization with those at the late follow-up showed that the frequency and magnitude of the responses had decreased significantly by time (proliferation/ELISPOT) ( P < 0.02) and shifted at the gene level from a Th1 to a Th2 profile ( P < 0.05). Conclusion: Id-specific T cells may decline overtime and shift toward a Th2 response and may be found at a similar frequency of patients in blood and BM.
- Subjects
VACCINATION; MULTIPLE myeloma; IMMUNIZATION; CELLULAR immunity; T cells; BONE marrow
- Publication
European Journal of Haematology, 2007, Vol 79, Issue 5, p371
- ISSN
0902-4441
- Publication type
Article
- DOI
10.1111/j.1600-0609.2007.00962.x