We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
MiR-7 Triggers Cell Cycle Arrest at the G1/S Transition by Targeting Multiple Genes Including Skp2 and Psme3.
- Authors
Sanchez, Noelia; Gallagher, Mark; Lao, Nga; Gallagher, Clair; Clarke, Colin; Doolan, Padraig; Aherne, Sinead; Blanco, Alfonso; Meleady, Paula; Clynes, Martin; Barron, Niall
- Abstract
MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27KIP and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti-apoptotic factors like p-Akt, promoted cell survival while arrested in G1. Thus miR-7 can co-ordinate the levels of multiple genes and proteins to influence G1 to S phase transition and the apoptotic response in order to maintain cellular homeostasis. This work provides further mechanistic insight into the role of miR-7 as a regulator of cell growth in times of cellular stress.
- Subjects
NON-coding RNA; CELL cycle; APOPTOSIS; DNA repair; GENE expression; GENETIC engineering; NUCLEIC acids; RNA interference
- Publication
PLoS ONE, 2013, Vol 8, Issue 6, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0065671