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- Title
Anti-inflammatory effect of glucose-mannose binding lectins isolated from Brazilian beans.
- Authors
Assreuy, A. M. S.; Shibuya, M. D.; Martins, G. J.; Souza, M. L. P. De; Cavada, B. S.; Moreira, R. A.; Oliveira, J. T. A.; Ribeiro, R. A.; Flores, C. A.
- Abstract
Selectins are essential for leukocyte recruitment in inflammation. Because of a lectin domain present in the selectin structure, we investigated the anti-inflammtory activity of six mannose glucose binding lectins from brazilian beans: Dioclea guianensis -DguiL; D. grandiflora -DgL; Cratylia floribunda -CfL; D. violacea -D.vL; D. virgata -DvirL and Canavalia brasiliensis -ConBr. The lectins were injected intravenously (i.v.) into rats (0.1 and 1.0 mg/kg; 30 min before irritants) and its activities compared to E. coli endotoxin (LPS,30 mug/kg i.v.). Three lectins (DvL, CfL and DguiL), although less intense than LPS, inhibited the neutrophil migration induced by carrageenan (Cg, 300 mug) in a dose-dependent manner (0.1 and 1.0 mg/kg). DvL activity was reversed by 0.1 M alphaD-methyl-mannoside (alpha-CH3), but not by 0.1 M alpha D-galactose. The fMLP (44 ng)-induced neutrophil migration was also reduced by these lectins. Endotoxin contamination of lectin samples could be excluded since alpha-CH3 treatment reversed the DvL effect, but did not modify LPS inhibitory activity. Carrageenan (300 mug)-induced paw oedema was also reduced by LPS or lectin treatments. Conversely, none of the tested lectins inhibited dextran (Dex, 300 mug)-induced paw oedema, a classical leukocyte independent model, or zymosan (Zy, 1.0 mg)-induced peritonitis and paw oedema. LPS showed no effect upon Dex-induced paw oedema and barely reduced (25%) the oedematogenic effects of zymosan. As proposed for LPS, the lectin inhibitory activity was better observed on neutrophil-mediated inflammatory reactions. We speculate that the plant lectin antiinflammatory activity is probably due to a competitive blockage of a common leukocyte and/or endothelial selectin carbohydrate ligand.
- Subjects
LECTINS; ANTI-inflammatory agents; INFLAMMATORY mediators
- Publication
Mediators of Inflammation, 1997, Vol 6, Issue 3, p201
- ISSN
0962-9351
- Publication type
Article
- DOI
10.1080/09629359791695