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- Title
Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.
- Authors
Şencan, İkbal; Esipova, Tatiana; Kılıç, Kıvılcım; Li, Baoqiang; Desjardins, Michèle; Yaseen, Mohammad A; Wang, Hui; Porter, Jason E; Kura, Sreekanth; Fu, Buyin; Secomb, Timothy W; Boas, David A; Vinogradov, Sergei A; Devor, Anna; Sakadžić, Sava
- Abstract
The cerebral cortex has a number of conserved morphological and functional characteristics across brain regions and species. Among them, the laminar differences in microvascular density and mitochondrial cytochrome c oxidase staining suggest potential laminar variability in the baseline O2 metabolism and/or laminar variability in both O2 demand and hemodynamic response. Here, we investigate the laminar profile of stimulus-induced intravascular partial pressure of O2 (pO2) transients to stimulus-induced neuronal activation in fully awake mice using two-photon phosphorescence lifetime microscopy. Our results demonstrate that stimulus-induced changes in intravascular pO2 are conserved across cortical layers I–IV, suggesting a tightly controlled neurovascular response to provide adequate O2 supply across cortical depth. In addition, we observed a larger change in venular O2 saturation (ΔsO2) compared to arterioles, a gradual increase in venular ΔsO2 response towards the cortical surface, and absence of the intravascular "initial dip" previously reported under anesthesia. This study paves the way for quantification of layer-specific cerebral O2 metabolic responses, facilitating investigation of brain energetics in health and disease and informed interpretation of laminar blood oxygen level dependent functional magnetic resonance imaging signals.
- Publication
Journal of Cerebral Blood Flow & Metabolism, 2022, Vol 42, Issue 3, p510
- ISSN
0271-678X
- Publication type
Article
- DOI
10.1177/0271678X20928011