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- Title
Human endometrial regenerative cells attenuate renal ischemia reperfusion injury in mice.
- Authors
Peng Sun; Jian Liu; Wenwen Li; Xiaoxi Xu; Xiangying Gu; HongYue Li; Hongqiu Han; Caigan Du; Hao Wang; Sun, Peng; Liu, Jian; Li, Wenwen; Xu, Xiaoxi; Gu, Xiangying; Li, HongYue; Han, Hongqiu; Du, Caigan; Wang, Hao
- Abstract
<bold>Background: </bold>Endometrial regenerative cells (ERCs) is an attractive novel type of adult mesenchymal stem cells that can be non-invasively obtained from menstrual blood and are easily replicated at a large scale without tumorigenesis. We have previously reported that ERCs exhibit unique immunoregulatory properties in experimental studies in vitro and in vivo. In this study, the protective effects of ERCs on renal ischemia-reperfusion injury (IRI) were examined.<bold>Methods: </bold>Renal IRI in C57BL/6 mice was induced by clipping bilateral renal pedicles for 30 min, followed by reperfusion for 48 h. ERCs were isolated from healthy female menstrual blood, and were injected (1 million/mouse, i.v.) into mice 2 h prior to IRI induction. Renal function, pathological and immunohistological changes, cell populations and cytokine profiles were evaluated after 48 h of renal reperfusion.<bold>Results: </bold>Here, we showed that as compared to untreated controls, administration of ERCs effectively prevented renal damage after IRI, indicated by better renal function and less pathological changes, which were associated with increased serum levels of IL-4, but decreased levels of TNF-α, IFN-γ and IL-6. Also, ERC-treated mice displayed significantly less splenic and renal CD4(+) and CD8(+) T cell populations, while the percentage of splenic CD4(+)CD25(+) regulatory T cells and infiltrating M2 macrophages in the kidneys were significantly increased in ERC-treated mice.<bold>Conclusions: </bold>This study demonstrates that the novel anti-inflammatory and immunoregulatory effects of ERCs are associated with attenuation of renal IRI, suggesting that the unique features of ERCs may make them a promising candidate for cell therapies in the treatment of ischemic acute kidney injury in patients.
- Subjects
ENDOMETRIAL diseases; REGENERATION (Biology); TREATMENT of reperfusion injuries; PHYSIOLOGICAL effects of cytokines; KIDNEY injuries; THERAPEUTICS; ANIMAL experimentation; ANTIGENS; CYTOKINES; ENDOMETRIUM; INFLAMMATION; ISCHEMIA; KIDNEYS; MACROPHAGES; MICE; NEUTROPHILS; REPERFUSION injury; SPLEEN; T cells
- Publication
Journal of Translational Medicine, 2016, Vol 14, p1
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-016-0782-3