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- Title
CD34 defines melanocyte stem cell subpopulations with distinct regenerative properties.
- Authors
Joshi, Sandeep S.; Tandukar, Bishal; Pan, Li; Huang, Jennifer M.; Livak, Ferenc; Smith, Barbara J.; Hodges, Theresa; Mahurkar, Anup A.; Hornyak, Thomas J.
- Abstract
Melanocyte stem cells (McSCs) are the undifferentiated melanocytic cells of the mammalian hair follicle (HF) responsible for recurrent generation of a large number of differentiated melanocytes during each HF cycle. HF McSCs reside in both the CD34+ bulge/lower permanent portion (LPP) and the CD34- secondary hair germ (SHG) regions of the HF during telogen. Using Dct-H2BGFP mice, we separate bulge/LPP and SHG McSCs using FACS with GFP and anti-CD34 to show that these two subsets of McSCs are functionally distinct. Genome-wide expression profiling results support the distinct nature of these populations, with CD34- McSCs exhibiting higher expression of melanocyte differentiation genes and with CD34+ McSCs demonstrating a profile more consistent with a neural crest stem cell. In culture and in vivo, CD34- McSCs regenerate pigmentation more efficiently whereas CD34+ McSCs selectively exhibit the ability to myelinate neurons. CD34+ McSCs, and their counterparts in human skin, may be useful for myelinating neurons in vivo, leading to new therapeutic opportunities for demyelinating diseases and traumatic nerve injury.
- Subjects
MELANOCYTES; STEM cells; NEURONS; NEURAL crest; NERVOUS system; TELOGEN
- Publication
PLoS Genetics, 2019, Vol 15, Issue 4, p1
- ISSN
1553-7390
- Publication type
Article
- DOI
10.1371/journal.pgen.1008034