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- Title
Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS).
- Authors
März, Anne; Mönch, Bettina; Rösch, Petra; Kiehntopf, Michael; Henkel, Thomas; Popp, Jürgen
- Abstract
In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.
- Subjects
METHYLTRANSFERASES; ERYTHROCYTES; SURFACE enhanced Raman effect; RAMAN spectroscopy; MICROFLUIDICS
- Publication
Analytical & Bioanalytical Chemistry, 2011, Vol 400, Issue 9, p2755
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-011-4811-z