We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Transgenic peach plants (Prunus persicaL.) produced by genetic transformation of embryo sections using the green fluorescent protein (GFP) as anin vivomarker.
- Authors
Pérez-Clemente, Rosa M.; Pérez-Sanjuán, Amparo; García-Férriz, Lorenzo; Beltrán, José-Pío; Cañas, Luis A.
- Abstract
The main obstacle to genetic engineering of fruit tree species is the regeneration of transformed plantlets. Transformation events in peach (Prunus persicaL.) have been reported using particle bombardment orAgrobacterium-mediated transformation of immature embryos. However, the regeneration of plants from transgenic tissues is still difficult and the recovery of non-chimeric plants has not been reported to date. In this paper we describe an efficient, reliable transformation and regeneration system to produce transgenic peach plants using embryo sections of mature seeds as starting material. This represents an important advantage due to the availability of such material throughout the year.A. tumefaciensstrain C58 (pMP90) containing the binary plasmid pBin19 was used as vector system for transformation. We used the Nospro-nptII-Noster cassette as a selectable marker and the CaMV35Spro-sgfp-CaMV35Ster cassette as a vital reporter gene coding for an improved version of the green fluorescent protein (sGFP).In vitrocultured embryo sections wereAgrobacterium-cocultivated and, after selection, transgenic shoots were regenerated. Shoots that survived exhibited high-level of sGFP expression mainly visible in the young leaves of the apex.In vivomonitoring of GFP expression permitted an early, rapid and easy discrimination of both transgenic and escape buds. After elimination of escapes, transgenic shoots were rootedin vitroand the recovered plantlets were screened using PCR amplification. Southern analysis confirmed stable genomic integration of thesgfptransgene. The high levels of GFP expression were also maintained in the second generation of transgenic peach plants.
- Subjects
PEACH; REGENERATION (Botany); TRANSGENIC plants; GENETIC transformation; PLANT breeding; PLANT genetics; GREEN fluorescent protein; PLANT embryology
- Publication
Molecular Breeding, 2004, Vol 14, Issue 4, p419
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-005-0506-5