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- Title
Production and secretion of a heterologous protein by turnip hairy roots with superiority over tobacco hairy roots.
- Authors
Huet, Yoann; Ekouna, Jean-Pierre; Caron, Aurore; Mezreb, Katiba; Boitel-Conti, Michèle; Guerineau, François
- Abstract
A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were constructed. One cassette was used to express the green fluorescent protein (GFP)-encoding gene in hairy roots grown in flasks. A stable and fast-growing hairy root line secreted GFP at >120 mg/l culture medium. GFP represented 60 % of the total soluble proteins in the culture medium. Turnip hairy roots retained sustainable growth and stable GFP production over 3 years. These results were superior to those obtained using tobacco hairy roots.
- Subjects
RHIZOBIUM rhizogenes; BRASSICA; GREEN fluorescent protein; PROTEINS; SECRETION
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 1, p181
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1335-y