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- Title
Isolation and characteristics of α-fibrinogenase venom from Naja oxiana.
- Authors
Yunusova, E.; Sadykov, E.; Shkinev, A.; Sultanalieva, N.
- Abstract
Venom of the Central Asian cobra ( Naja oxiana Eichwald, NO) was shown to contain proteinase activity that cleaved the fibrinogen α-chain. An electrophoretically homogeneous preparation of basic (pI ~ 10) α-fibrinogenase (α-Fg) with MW 60 kDa exhibited the maximum activity at pH 6.0-8.0. α-Fg was a metalloproteinase (activity suppressed only by EDTA) that was thermolabile; at optimum temperature of 20-40°C (heating for 15 min at 60°C led to an activity loss of 80-85%). The enzyme cleaved dose-dependently the Aα-chain. The α-chain of human fibrinogen was hydrolyzed completely in 20 min with an experimental dose of 15 μg.
- Subjects
THROMBIN; VENOM; NAJA oxiana; FIBRINOGEN; TEMPERATURE
- Publication
Chemistry of Natural Compounds, 2013, Vol 49, Issue 4, p712
- ISSN
0009-3130
- Publication type
Article
- DOI
10.1007/s10600-013-0714-x