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- Title
Polysaccharides from Capsosiphon fulvescens Stimulate the Growth of IEC-6 Cells by Activating the MAPK Signaling Pathway.
- Authors
Go, Hiroe; Hwang, Hye-Jung; Nam, Taek-Jeong
- Abstract
Seaweed extracts show diverse bioactivities, such as antioxidant and antitumor activity. Capsosiphon fulvescens is a green alga that is abundant along the southwest coast of South Korea. Although it is consumed for its purported health-enhancing properties, particularly as a treatment for stomach disorders and hangovers, the health effects of dietary C. fulvescens remain unclear. We extracted polysaccharides from C. fulvescens ( Cf-PS), investigated their effects on the proliferation of rat small intestinal epithelial IEC-6 cells, and determined the signaling cascade involved. We cultured IEC-6 cells in the presence of Cf-PS, which stimulated cell proliferation in a dose-dependent manner, and analyzed the Wnt and MAPK signaling pathways, which are related to cell proliferation. Cf-PS treatment induced the translocation of β-catenin, an effector of the Wnt signaling pathway, from the cytosol to the nucleus and increased the expression of cyclinD1 and c-myc. Cf-PS also induced ERK1/2 phosphorylation, which is activated by mitogenic and proliferative stimuli such as growth factors, but the phosphorylation of JNK and p38 was not enhanced. Our results show that Cf-PS regulates proliferation via stimulating the nuclear translocation of β-catenin and ERK1/2 activation in intestinal epithelial cells.
- Publication
Marine Biotechnology, 2011, Vol 13, Issue 3, p433
- ISSN
1436-2228
- Publication type
Article
- DOI
10.1007/s10126-010-9314-y