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- Title
Establishment, characterization and drug sensitivity testing in primary cultures of human thymoma and thymic carcinoma.
- Authors
Ehemann, Volker; Kern, Michael A.; Breinig, Marco; Schnabel, Philipp A.; Gunawan, Bastian; Schulten, Hans-Jörg; Schlaeger, Christoph; Radlwimmer, Bernhard; Steger, Christina M.; Dienemann, Hendrik; Lichter, Peter; Schirmacher, Peter; Rieker, Ralf J.
- Abstract
Thymomas and thymic carcinomas are peculiar epithelial tumors of the anterior mediastinum. They may show aggressive clinical behavior and are a paradigm for the interaction between the tumor and the immune system. So far, adequate functional studies enabling a better understanding of this malignancy have not been performed, since human thymoma/thymic carcinoma cell lines have not been available. Here, the authors describe the establishment, characterization and functional analyses of epithelial cell lines from a Type B1-thymoma and a poorly differentiated thymic carcinoma. By Fluorescence-activated cell sorting (FACS) analyses, both cell lines were aneuploid. The aneuploid cell fraction of the thymic carcinoma cell line was characterized by a high proliferation index of 55.9%, in contrast to a lower proliferation rate of the aneuploid cell fraction of the thymoma (19.7%). Array-based comparative genomic hybridization (aCGH) and conventional cytogenetic analysis of the thymoma revealed only minor imbalances whereas the thymic carcinoma was characterized by a complex karyotype in the hyperdiploid range that was readily defined with multicolor FISH (mFISH). Application of a selective COX-2 inhibitor reduced cell viability in both cell lines in a dose-dependent manner. In conclusion, these first cell lines of a thymoma and a CD5-positive thymic carcinoma are useful tools for further in vitro studies of cellular, molecular and genetic aspects of the disease and for functional tests to evaluate new therapeutic targets. © 2008 Wiley-Liss, Inc.
- Publication
International Journal of Cancer, 2008, Vol 122, Issue 12, p2719
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.23335