We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17.
- Authors
Chrast, Lukas; Tratsiak, Katsiaryna; Planas-Iglesias, Joan; Daniel, Lukas; Prudnikova, Tatyana; Brezovsky, Jan; Bednar, David; Kuta Smatanova, Ivana; Chaloupkova, Radka; Damborsky, Jiri
- Abstract
Haloalkane dehalogenases are enzymes with a broad application potential in biocatalysis, bioremediation, biosensing and cell imaging. The new haloalkane dehalogenase DmxA originating from the psychrophilic bacterium Marinobacter sp. ELB17 surprisingly possesses the highest thermal stability (apparent melting temperature Tm,app = 65.9 °C) of all biochemically characterized wild type haloalkane dehalogenases belonging to subfamily II. The enzyme was successfully expressed and its crystal structure was solved at 1.45 Å resolution. DmxA structure contains several features distinct from known members of haloalkane dehalogenase family: (i) a unique composition of catalytic residues; (ii) a dimeric state mediated by a disulfide bridge; and (iii) narrow tunnels connecting the enzyme active site with the surrounding solvent. The importance of narrow tunnels in such paradoxically high stability of DmxA enzyme was confirmed by computational protein design and mutagenesis experiments.
- Subjects
PSYCHROPHILIC bacteria; DEHALOGENASES; BINDING sites; PROTEIN engineering; CELL imaging
- Publication
Microorganisms, 2019, Vol 7, Issue 11, p498
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms7110498