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- Title
High Prevalence of Staphylococcal Enterotoxins (SEs) Virulence Genes Among Methicillin-Resistant Staphylococcus aureus Isolated from Patients with Different Clinical Manifestations in Khartoum State, Sudan.
- Authors
Hamdan, Elsadig M.; Hassan, Asmaa A.; Amin, Safa A.; Ahmed, Hiba A.; Ataelmanan, Amany E.; Elhassan, Mogahid M.
- Abstract
Background: Staphylococcal enterotoxins (SEs) are family members of more than 20 different staphylococcal and streptococcal exotoxins. SEA and SEB are the most common toxins in staphylococcus-related food poisoning. Objectives The present study aimed to estimate the prevalence of SEs Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from different clinical samples. Methods: A cross-sectional laboratory-based study was conducted from November 2020 to January 2021. To isolate and identify S. aureus, the conventional method and protein A latex test were used. MRSA was detected using methicillin (MET 5μg) by using the Kirby-Baur disk diffusion method. Extracted DNA was amplified for the specific Staphylococcal enterotoxins (SE) gene to confirm the SEA, SEB and SEC. Result: The SEA gene was observed among 120 (100%) clinical isolates. The combined staphylococcal enterotoxin genes A, B and C were found in 33.3% of the isolates. Respiratory tract infection (RTI) shows the highest percentage of SEA 28.3%. Conclusion: The present study showed a high prevalence of SEA in clinical settings at Khartoum State. A high prevalence of the combination of more than one gene occur in SEA with SEC, which was represented 82.5%. The present study showed a strong relationship between MRSA and SEA that could be due to a specific strain of MRSA carrying the SEA gene. Further studies should be done about Staphylococcal enterotoxins.
- Subjects
KHARTOUM (Sudan); SUDAN; METHICILLIN-resistant staphylococcus aureus; ENTEROTOXINS; SYMPTOMS; RESPIRATORY infections; FOOD poisoning; MUPIROCIN; EXOTOXIN
- Publication
Egyptian Academic Journal of Biological Sciences, G, Microbiology, 2022, Vol 14, Issue 1, p77
- ISSN
2090-0872
- Publication type
Article
- DOI
10.21608/EAJBSG.2022.226663