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- Title
First Chromosome-Scale Assembly and Deep Floral-Bud Transcriptome of a Male Kiwifruit.
- Authors
Tahir, Jibran; Crowhurst, Ross; Deroles, Simon; Hilario, Elena; Deng, Cecilia; Schaffer, Robert; Le Lievre, Liam; Brendolise, Cyril; Chagné, David; Gardiner, Susan E.; Knaebel, Mareike; Catanach, Andrew; McCallum, John; Datson, Paul; Thomson, Susan; Brownfield, Lynette R.; Nardozza, Simona; Pilkington, Sarah M.
- Abstract
Genome and RNA Sequencing Pacific BioSciences (Menlo Park, California, United States) Sequel Single Molecule Real-Time (SMRT) sequencing technology was used to generate long-read data from "Russell" nuclear DNA for whole-genome assembly at the Genetics Laboratory, Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia. Primary contigs were assigned to linkage groups using information from a genetic map previously described ([35]) with additional input from DNA-based synteny to other whole genome sequences for I Actinidia i species as follows: DNA markers ([35]) were aligned with the "Russell" contigs using Bowtie2 ([19]) (version 2.3.4.3). Keywords: male kiwifruit genome; sex determining region; long read genome assembly; transcriptomic of male flower; PacBio and illumina sequencing; male - genetics; whole-genome sequencing (WGS) EN male kiwifruit genome sex determining region long read genome assembly transcriptomic of male flower PacBio and illumina sequencing male - genetics whole-genome sequencing (WGS) 1 8 8 05/19/22 20220516 NES 220516 Introduction Over 50 kiwifruit species from the genus I Actinidia i represent climbing perennial vines that show variable hardiness and dioecy and produce fruits with a delicious flavor ([15]). Placement of contigs for which no markers exist was based on the consensus of DNA synteny-based comparison to published I Actinidia i genomes ([35]; [41]; [44]) and to the long-read-based genome assembly for Red5 V2 ([36]).
- Subjects
KIWIFRUIT; CELLULOSE synthase; LOCUS (Genetics); Y chromosome; GENOMICS; TRANSCRIPTOMES; GENE families; LYCOPENE
- Publication
Frontiers in Genetics, 2022, Vol 13, p1
- ISSN
1664-8021
- Publication type
Article
- DOI
10.3389/fgene.2022.852161