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- Title
Genotypic Polymorphisms of Hepatitis B Virus Provide Useful Information for Estimating Geographical Origin or Place of Long-term Residence of Unidentified Cadavers.
- Authors
Inoue, Hiroyuki; Motani‐Saitoh, Hisako; Sakurada, Koichi; Ikegaya, Hiroshi; Yajima, Daisuke; Nagasawa, Sayaka; Iwase, Hirotaro
- Abstract
Increasing numbers of unidentified cadavers are a major problem. We have developed a new method for providing identification information that can determine the geographical origin or place of long-term residence of unidentified cadavers based on genotypic polymorphisms of hepatitis B virus ( HBV) known to correlate with their geographical distribution. PCR of serum samples detected HBV DNA from 4 (3.9%) of 102 randomly selected Japanese forensic cadavers. Multiplex PCR did not detect multiple HBV genotypes from any single cadaver, confirming the absence of coinfection. Phylogenetic tree analysis based on a 485-bp mutant region of the HBV S gene successfully classified the HBV genotypes into A to J. Among 10 HBV-infected cadavers, 8 had genotype Ce/C2, a genotype prevalent in East Asia, and 2 had genotype Bj/B1, a Japanese-specific genotype. HBV genotypic polymorphisms correlate with the geographical distribution of the virus and thus provide important information for identifying unidentified cadavers infected with HBV.
- Subjects
GENETIC polymorphisms; HEPATITIS B virus; VIRAL genetics; IDENTIFICATION of the dead; POLYMERASE chain reaction; FORENSIC sciences; SERUM
- Publication
Journal of Forensic Sciences, 2014, Vol 59, Issue 1, p236
- ISSN
0022-1198
- Publication type
Article
- DOI
10.1111/1556-4029.12257