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- Title
Single-cell profiling reveals a memory B cell-like subtype of follicular lymphoma with increased transformation risk.
- Authors
Wang, Xuehai; Nissen, Michael; Gracias, Deanne; Kusakabe, Manabu; Simkin, Guillermo; Jiang, Aixiang; Duns, Gerben; Sarkozy, Clementine; Hilton, Laura; Chavez, Elizabeth A.; Segat, Gabriela C.; Wong, Rachel; Kim, Jubin; Aoki, Tomohiro; Islam, Rashedul; May, Christina; Hung, Stacy; Tyshchenko, Kate; Brinkman, Ryan R.; Hirst, Martin
- Abstract
Follicular lymphoma (FL) is an indolent cancer of mature B-cells but with ongoing risk of transformation to more aggressive histology over time. Recurrent mutations associated with transformation have been identified; however, prognostic features that can be discerned at diagnosis could be clinically useful. We present here comprehensive profiling of both tumor and immune compartments in 155 diagnostic FL biopsies at single-cell resolution by mass cytometry. This revealed a diversity of phenotypes but included two recurrent patterns, one which closely resembles germinal center B-cells (GCB) and another which appears more related to memory B-cells (MB). GCB-type tumors are enriched for EZH2, TNFRSF14, and MEF2B mutations, while MB-type tumors contain increased follicular helper T-cells. MB-type and intratumoral phenotypic diversity are independently associated with increased risk of transformation, supporting biological relevance of these features. Notably, a reduced 26-marker panel retains sufficient information to allow phenotypic profiling of future cohorts by conventional flow cytometry.Follicular lymphoma can transform to a more aggressive histology. Here, the authors use bulk and single cell analysis to create a 26 marker panel which could be used to profile FL samples and predict the risk of transformation using flow cytometry.
- Publication
Nature Communications, 2022, Vol 13, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-022-34408-0