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- Title
Maternal Plasma DNA Sequencing Reveals the Genome-Wide Genetic and Mutational Profile of the Fetus.
- Authors
Dennis Lo, Y. M.; Allen Chan, K. C.; Hao Sun; Chen, Eric Z.; Peiyong Jiang; Lun, Fiona M. F.; Zheng, Yama W.; Leung, Tak Y.; Lau, Tze K.; Cantor, Charles R.; Chiu, Rossa W. K.
- Abstract
Cell-free fetal DNA is present in the plasma of pregnant women. It consists of short DNA fragments among primarily maternally derived DNA fragments. We sequenced a maternal plasma DNA sample at up to 65-fold genomic coverage. We showed that the entire fetal and maternal genomes were represented in maternal plasma at a constant relative proportion. Plasma DNA molecules showed a predictable fragmentation pattern reminiscent of nuclease-cleaved nucleosomes, with the fetal DNA showing a reduction in a 166-base pair (bp) peak relative to a 143-bp peak, when compared with maternal DNA. We constructed a genome-wide genetic map and determined the mutational status of the fetus from the maternal plasma DNA sequences and from information about the paternal genotype and maternal haplotype. Our study suggests the feasibility of using genome-wide scanning to diagnose fetal genetic disorders prenatally in a noninvasive way.
- Publication
Science Translational Medicine, 2010, Vol 2, Issue 61, p1
- ISSN
1946-6234
- Publication type
Article
- DOI
10.1126/scitranslmed.3001720