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- Title
Development and Validation of Liquid Chromatographic Method for the Determination of Zolmitriptan in Pharmaceutical Formulations.
- Authors
Srinivasu, Topalli; Annapurna, M. Mathrusri; Chandrasekhar, T. G.
- Abstract
A simple, precise, accurate and robust high-performance liquid chromatographic method was developed and validated for the determination of Zolmitriptan in tablet dosage forms. Reversed-phase chromatography was performed on X Bridge RP-18 (150 mm × 4.6 mm, 3.5 μm) column of Waters HPLC with Empower software with photodiode array detector, maintained at 25°C. Ammonium bicarbonate buffer (10 mM): Acetonitrile (90:10, v/v) was used as mobile phase at a flow rate of 1 mL min-1 with UV detection at 220 nm. Linearity was observed in the concentration range of 0.1-250 μg mL-1 with regression equation y = 32567 x + 9883.8 (R2 = 0.9999). The limit of quantitation (LOQ) and limit of detection (LOD) were found to be 0.064 and 0.019 μg mL-1 respectively. The method was validated as per ICH guidelines. The RSD for intra-day (0.22-0.36) and inter-day (0.52-0.79) precision were found to be less than 1 %. The percentage recovery was in good agreement with the labeled amount in the pharmaceutical formulations and the method is simple, precise, accurate and robust for the determination of Zolmitriptan in pharmaceutical formulations.
- Subjects
LIQUID chromatography; DRUG tablets; HYDROPHILIC interaction liquid chromatography; PHOTODIODES; AMMONIUM bicarbonate; BUFFER solutions
- Publication
Drug Invention Today, 2012, Vol 4, Issue 9, p450
- ISSN
0975-7619
- Publication type
Article