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- Title
Allergic arthritis induced by cationic antigens: relationship of chronicity with antigen retention and T-cell reactivity.
- Authors
Van Lent, P. L. E. M.; Van Den Berg, W. B.; Schalkwijk, J.; Van De Putte, L. B. A.; Van Den Bersselaar, L.
- Abstract
in order to define the antigenic properties necessary for sustained allergic arthritis, we prepared a range of differently charged bovine serum albumin (BSA) species with increasing isoelectric points (4.5, 4.5-7.4, 7-8, 85-9 and >9). The highly cationic BSA >9 appeared to be in a polymeric form. We investigated three properties of these proteins: (i) antigen retention, (ii) T-cell reactivity, and (iii) arthritis induction. Injection of the respective radiolabelled antigens in the knee-joints of immunized mice showed that antigen retention increased with cationicity of the proteins, with the best retention found for ESA with pI>9. However, sustained joint inflammation was only found with BSA859, and not with a level RSA of lower or even higher pI T-cell reactivity in vivo as measured by delayed-type hypersensitivity (skin testing) was similar for the tested antigens, with the exception of polymeric BSA (>9). The latter appeared to be a poor antigen. In vitro, T-cell reactivity ([³H]- thymidine incorporation) against the cationized BSA species was slightly higher as compared to native BSA. The combination of excellent antigen retention and adequate T-cell reactivity appears to be optimal for the induction of chronic arthritis.
- Subjects
ARTHRITIS; ALLERGIES; ANTIGENS; T cells; SERUM albumin; IMMUNITY
- Publication
Immunology, 1987, Vol 62, Issue 2, p265
- ISSN
0019-2805
- Publication type
Article