We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.
- Authors
Bienko, Magda; Crosetto, Nicola; Teytelman, Leonid; Klemm, Sandy; Itzkovitz, Shalev; van Oudenaarden, Alexander
- Abstract
We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.
- Subjects
GENOMES; POLYMERASE chain reaction; FISH DNA; LOCUS (Genetics); PIPELINES; GENE targeting; TRANSCRIPTION factors; CHROMOSOMES
- Publication
Nature Methods, 2013, Vol 10, Issue 2, p122
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2306