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- Title
Live-cell 3D super-resolution imaging in thick biological samples.
- Authors
Cella Zanacchi, Francesca; Lavagnino, Zeno; Perrone Donnorso, Michela; Del Bue, Alessio; Furia, Laura; Faretta, Mario; Diaspro, Alberto
- Abstract
We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 ?m), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.
- Subjects
FLUORESCENCE microscopy; THREE-dimensional imaging in biology; CELL culture; HIGH resolution imaging; SIGNAL-to-noise ratio
- Publication
Nature Methods, 2011, Vol 8, Issue 12, p1047
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1744