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- Title
Antibacterial properties of chicken intestinal phospholipase A2.
- Authors
Karray, Aida; Ali, Yassine Ben; Gargouri, Youssef; Bezzine, Sofiane
- Abstract
Background: The presence of chicken group-IIA PLA2 (ChPLA2-IIA) in the intestinal secretion suggests that this enzyme plays an important role in systemic bactericidal defence. We have analyzed the bactericidal activity of purified ChPLA2-IIA, on several gram-positive and gram-negative bacteria by using the diffusion well and dilution methods. Results: ChPLA2-IIA displays potent bactericidal activity against gram-positive bacteria but lacks bactericidal activity against gram negative ones. We have also demonstrated a synergic action of ChPLA2-IIA with lysozyme when added to the bacteria culture prior to ChPLA2-IIA. The bactericidal efficiency of ChPLA2-IIA was shown to be dependent upon the presence of calcium ions and then a correlation could be made to its hydrolytic activity of membrane phospholipids. Interestingly ChPLA2-IIA displays a higher dependence to Ca2+ ions than to Mg2+ions. Conclusion: We conclude that the main physiological role of ChPLA2-IIA could be the defence of the intestine against bacterial invasions.
- Subjects
PHOSPHOLIPASE A2; BACTERIA; CALCIUM ions; INTESTINAL secretion; ENZYMES
- Publication
Lipids in Health & Disease, 2011, Vol 10, Issue 1, p1
- ISSN
1476-511X
- Publication type
Article
- DOI
10.1186/1476-511X-10-4