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- Title
The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barré syndrome.
- Authors
Godschalk, Peggy C. R.; Heikema, Astrid P.; Gilbert, Michel; Komagamine, Tomoko; Ang, C. Wim; Glerum, Jobine; Brochu, Denis; Li, Jianjun; Yuki, Nobuhiro; Jacobs, Bart C.; van Belkum, Alex; Endtz, Hubert P.
- Abstract
Molecular mimicry of Campylobacter jejuni Iipo-oligosaccharides (LOS) with gangliosides in nervous tissue is considered to induce cross-reactive antibodies that lead to Guillain-Barré syndrome (GBS), an acute polyneuropathy. To determine whether specific bacterial genes are crucial for the biosynthesis of ganglioside-like structures and the induction of anti-ganglioside antibodies, we characterized the C.jejuni LOS biosynthesis gene locus in GBS -associated and control strains. We demonstrated that specific types of the LOS biosynthesis gene locus are associated with GBS and with the expression of ganglioside-mimicking structures. Campylobacter knockout mutants of 2 potential GBS marker genes, both involved in LOS sialylation, expressed truncated LOS structures without sialic acid, showed reduced reactivity with GBS patient serum, and failed to induce an anti-ganglioside antibody response in mice. We demonstrate, for the first time, to our knowledge, that specific bacterial genes are crucial for the induction of anti-ganglioside antibodies.
- Subjects
CAMPYLOBACTER; NERVE tissue; IMMUNOGLOBULINS; GENES; BIOSYNTHESIS; MICE
- Publication
Journal of Clinical Investigation, 2004, Vol 114, Issue 11, p1659
- ISSN
0021-9738
- Publication type
Article
- DOI
10.1172/JCI200415707