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- Title
Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl-2 expression after permanent focal ischemia in rats.
- Authors
Bian Jieyong; Wang Zhong; Zhang Shiming; Zhou Dai; Yoko Kato; Tetsuo Kanno; Hirotoshi Sano
- Abstract
Both mild hypothermia (MH) and decompressive craniectomy (CE) have been shown to have neuroprotective effects in brain ischemia. We investigated a possible effect of MH and a combination of CE and MH (CE+MH) on the changes of infarction size, DNA fragmentation, and immunoreactivities for Bcl-2 and Bax after 24 h of permanent middle cerebral artery occlusion (MCAO) in rats. For the estimation of ischemic brain injury, we calculated the infarct size of the MCA region at 24 h after the MCAO. Terminal deoxynucleotidyl transferase-mediated dUTP–biotin in situ nick labeling (TUNEL) staining was performed for the detection of DNA fragmentation. Immunoreactivities for Bcl-2 and Bax were stained. Infarction size after permanent MCAO was significantly reduced by CE+MH treatment ( P<0.01). Infarction size did not change significantly by application of MH alone ( P>0.05). TUNEL staining was remarkably reduced both in MH-treated animals and in CE+MH-treated animals. Immunoreactivity for Bcl-2 was greatly induced both in MH-treated animals and in CE+MH-treated animals. Induction of immunoreactivity for Bcl-2 was obviously inhibited both in MH-treated animals and in CE+MH-treated animals. It suggests that temporary MH delays infarct evolution and ameliorates neuron apoptosis but does not significantly reduce definite infarction size. CE+MH not only ameliorates neuron apoptosis but also remarkably reduces infarction size.
- Subjects
SKULL surgery; HYPOTHERMIA; CEREBRAL ischemia; APOPTOSIS; DNA; GENES; BRAIN blood-vessels
- Publication
Neurosurgical Review, 2006, Vol 29, Issue 2, p168
- ISSN
0344-5607
- Publication type
Article
- DOI
10.1007/s10143-005-0010-8