We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Enzymatic polymerization of dihydroquercetin using bilirubin oxidase.
- Authors
Khlupova, M.; Vasil'eva, I.; Shumakovich, G.; Morozova, O.; Chertkov, V.; Shestakov, A.; Kisin, A.; Yaropolov, A.
- Abstract
Dihydroquercetin (or taxifolin) is one of the most famous flavonoids and is abundant in Siberian larch ( Larix sibirica). The oxidative polymerization of dihydroquercetin (DHQ) using bilirubin oxidase as a biocatalyst was investigated and some physicochemical properties of the products were studied. DHQ oligomers (oligoDHQ) with molecular mass of 2800 and polydispersity of 8.6 were obtained by enzymatic reaction under optimal conditions. The oligomers appeared to be soluble in dimethylsulfoxide, dimethylformamide, and methanol. UV-visible spectra of oligoDHQ in dimethylsulfoxide indicated the presence of highly conjugated bonds. The synthesized oligoDHQ was also characterized by FTIR and H and C NMR spectroscopy. Comparison of NMR spectra of oligoDHQ with DHQ monomer and the parent flavonoids revealed irregular structure of a polymer formed via the enzymatic oxidation of DHQ followed by nonselective radical polymerization. As compared with the monomer, oligoDHQ demonstrated higher thermal stability and high antioxidant activity.
- Subjects
BILIRUBIN oxidase; QUERCETIN derivatives; POLYMERIZATION; ENZYMATIC analysis; FLAVONOIDS; SIBERIAN larch; ANTIOXIDANT analysis
- Publication
Biochemistry (00062979), 2015, Vol 80, Issue 2, p233
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297915020108