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- Title
Removal of mature bacterial biofilm from oval canals promoted by different final irrigation protocols.
- Authors
CINTRA, Fernanda; Martins NASCIMENTO, Wayne; Carvalho PRADO, Marina; Cristina SACOMANI, Arieth; Aguiar CAMPOS, Ana Elisa; DE-JESUS-SOARES, Adriana; FROZONI, Marcos
- Abstract
Objective: This study evaluated the effectiveness of final irrigation protocols performed with conventional irrigation, XP-Endo Finisher (FKG Dentaire, Switzerland) and Easy Clean (Easy Dental Equipment, Brazil) in the removal of intracanal mature bacterial biofilm. Methods: Mandibular incisors with oval shaped-canals were selected and contaminated with a pure culture of Enterococcus faecalis ATCC 29212 for 21 days at 37º C for the formation of a mature biofilm. After incubation period, scanning electron microscopy (SEM) was performed to confirm biofilms formation on root canal walls. Teeth were randomly distributed into 3 groups (n=15), according to the final irrigation protocol: conventional irrigation with syringe and cannula, XP-Endo Finisher and Easy Clean. The intracanal bacteriological samples were taken before (S1) and after (S2) preparation and the number of colony-forming units per milliliter (CFU/ml) was calculated. T-test and ANOVA with Tukey post-hoc were respectively used for intragroup and intergroup analysis. Significance level was stablished at p<0.05. Results: Successful biofilm formation was confirmed by SEM. All techniques significantly reduced bacterial colonies after the irrigation protocol (S2) in comparison with initial values (S1) (p<0.001). No statistic difference was observed between the protocols evaluated (p>0.05). Conclusion: The final irrigation protocols with XP-Endo Finisher and Easy Clean presented similar levels of bacterial removal in oval-shaped canals. However, these instruments did not reduce the amount of biofilms compared to the protocol of conventional irrigation with syringe and cannula.
- Subjects
IRRIGATION (Medicine); BIOFILMS
- Publication
Dental Press Endodontics, 2024, Vol 14, Issue 1, p92
- ISSN
2178-3713
- Publication type
Article
- DOI
10.14436/2358-2545.14.1.092-115.oar