We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Development of surface plasmon resonance immunosensor for the novel protein immunostimulating factor.
- Authors
Chen, Hongxia; Lee, Jaebeom; Jo, Wol-Soon; Jeong, Min-Ho; Koh, Kwangnak
- Abstract
The immunostimulating factor (ISTF) plays an important role in immunopathologic changes associated with Actinobacillus actinomycetemcomitans where it is located in the outer cell membrane. We describe a novel method by which ISTF can be monitored using a protein chip system based on surface plasmon resonance (SPR). The affinity of ISTF to its monoclonal antibodies and other proteins was compared. In fabrication of an ISTF immunosensor, a calix[4]crown ether monolayer was anchored onto a gold surface for use as an artificial protein linker system, and then characterized by Fourier Transform infrared reflection absorption spectroscopy, atomic force microscopy, and cyclic voltammetry. Anti-ISTF was immobilized onto the monolayer, and the interaction between ISTF and its antibodies was investigated by SPR. The calix[4]crown ether was found to assemble as a monolayer on the gold surface. Orientation and accessibility of anti-ISTF were assessed by the selective binding of ISTF. Modification of the SPR chip with the calix[4]crown monolayer provides a reliable and simple experimental platform for investigation of isolated proteins under experimental conditions resembling those of their native environment. [Figure not available: see fulltext.]
- Subjects
BIOSENSORS; SURFACE plasmon resonance; PROTEINS; IMMUNOPATHOLOGY; MOLECULAR self-assembly; MONOMOLECULAR films; MONOCLONAL antibodies; CELL membranes
- Publication
Microchimica Acta, 2011, Vol 172, Issue 1/2, p171
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-010-0476-0