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- Title
Effect of MTHFR 677C>T on plasma total homocysteine levels in renal graft recipients.
- Authors
FÖDINGER, MANUELA; WÖLFL, GABRIELE; FISCHER, GOTTFRIED; RASOUL-ROCKENSCHAUB, SUSANNE; SCHMID, RAINER; HÖRL, WALTER H.; SUNDER-PLASSMANN, GERE; Sunder-Plassmann, Gere
- Abstract
Effect ofMTHFR 677C>T on plasma total homocysteine levels in renal graft recipients. Background. Hyperhomocysteinemia is an established, independent risk factor for vascular disease morbidity and mortality. The 5,10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T has been shown to result in increased total homocysteine concentrations on the basis of low folate levels caused by a decreased enzyme activity. The effect of this polymorphism on total homocysteine and folate plasma levels in renal transplant patients is unknown. Methods. We screened 636 kidney graft recipients for the presence of the MTHFR C677T gene polymorphism. The major determinants of total homocysteine and folate plasma concentrations of 63 patients, who were identified to be homozygous for this gene polymorphism compared with heterozygotes (N = 63), and patients with wild-type alleles (N = 63), who were matched for sex, age, glomerular filtration rate (GFR), and body mass index, were identified by analysis of covariance. The variables included sex, age, GFR, body mass index, time since transplantation, folate and vitamin B12 levels, the use of azathioprine, and the MTHFR genotype. To investigate the impact of the kidney donor MTHFR genotype on total homocysteine and folate plasma concentrations, a similar model was applied in 111 kidney graft recipients with stable graft function, in whom the kidney donor C677T MTHFR gene polymorphism was determined. Results. The allele frequency of the C677T polymorphism in the MTHFR gene was 0.313 in the whole study population [wild-type (CC), 301; heterozygous (CT), 272; and homozygous mutant (TT), 63 patients, respectively] and showed no difference in the patient subgroups with various renal diseases. The MTHFR C677T gene polymorphism significantly influenced total homocysteine and folate plasma concentrations in renal transplant recipients (P = 0.0009 and P = 0.0002, respectively). Furthermore, a significant influence of the...
- Subjects
HOMOCYSTEINE; KIDNEY transplantation; GENETIC polymorphisms; FOLIC acid
- Publication
Kidney International, 1999, Vol 55, Issue 3, p1072
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.1999.0550031072.x