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- Title
Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke.
- Authors
Acquadro, Alberto; Lanteri, Sergio; Scaglione, Davide; Arens, Paul; Vosman, Ben; Portis, Ezio
- Abstract
Cynara cardunculus includes three taxa, the globe artichoke (subsp. scolymus L. Hegi), the cultivated cardoon (var. altilis) and their progenitor, the wild cardoon (var. sylvestris). Globe artichoke is an important component of the Mediterranean rural economy, but its improvement through breeding has been rather limited and its genome organization remains largely unexplored. Here, we report the isolation of 61 new microsatellite loci which amplified a total of 208 alleles in a panel of 22 C. cardunculus genotypes. Of these, 51 were informative for linkage analysis and 39 were used to increase marker density in the available globe artichoke genetic maps. Sequence analysis of the 22 loci associated with genes showed that 9 are located within coding sequence, with the repetitive domain probably being involved in DNA binding or in protein–protein interactions. The expression of the genes associated with 9 of the 22 microsatellite loci was demonstrated by RT-PCR.
- Subjects
ARTICHOKES; CARDOON; GENOMES; MICROSATELLITE repeats; GENE mapping
- Publication
Theoretical & Applied Genetics, 2009, Vol 118, Issue 8, p1573
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-009-1005-6