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- Title
Investigation of a Thermoplastic Polymeric Carrier for Bone Tissue Engineering Using Allogeneic Mesenchymal Stem Cells in Granular Scaffolds.
- Authors
Mylonas, Dimitrios; Vidal, Miguel D.; De Kok, Ingeborg J.; Moriarity, John D.; Cooper, Lyndon F.
- Abstract
: The purpose of this project was to compare alveolar bone repair by allogeneic mesenchymal stem cells using bioglass or synthetic hydroxyapatite (HA)/tricalcium phosphate (TCP) granular scaffolds delivered in a thermoplastic polymeric carrier. : Canine mesenchymal stem cells were obtained from iliac crest bone marrow of beagle dogs and expanded without differentiation. Cells were resuspended at a final concentration of 5 × 106 cells/ml in a thermoplastic polymeric carrier (30% w/v Pluronic F-127) and mixed with an equal volume of synthetic HA/TCP or bioglass scaffold and placed into surgically created 5 mm cylindrical defects in the edentulous premolar region of beagle dogs. After 4 weeks or 7 weeks, tissue healing was evaluated by standard histomorphometric methods (Bioquant Nova, Bioquant Image Analysis Corporation, Nashville, TN) by measurement of bone formation within five random sites from each biopsy. : After 4 weeks, sites treated with or without mesenchymal stem cells contained 58.25 ±18.43% or 43.35 ± 17.68% bone area ( p= 0.049), respectively. After 7 weeks, sites treated with or without mesenchymal stem cells contained 62.73 ± 19.10% or 60.39 ± 21.32% bone area. Bone formation occurred without inflammation in defects treated using Pluronic F-127 carrier with and without mesenchymal stem cells. There was no difference in percent bone area when bioglass or HA/TCP scaffolds were compared at either time point. The thermoplastic polymeric carrier did not limit alveolar bone repair in the canine mandible. The combination of a thermoplastic, viscous carrier with a granular scaffold allowed for the delivery of allogeneic mesenchymal stem cells in a clinically manageable form that enhanced bone formation at early stages of alveolar repair.
- Subjects
ALVEOLAR process; SYNTHETIC gums &; resins; THERMOPLASTICS; ALVEOLAR nerve; HYDROXYAPATITE coating
- Publication
Journal of Prosthodontics, 2007, Vol 16, Issue 6, p421
- ISSN
1059-941X
- Publication type
Article
- DOI
10.1111/j.1532-849X.2007.00218.x