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- Title
Origins and clonal convergence of gastrointestinal IgE<sup>+</sup> B cells in human peanut allergy.
- Authors
Hoh, Ramona A.; Joshi, Shilpa A.; Lee, Ji-Yeun; Martin, Brock A.; Varma, Sushama; Kwok, Shirley; Nielsen, Sandra C. A.; Nejad, Parastu; Haraguchi, Emily; Dixit, Priya S.; Shutthanandan, Swetha V.; Roskin, Krishna M.; Zhang, Wenming; Tupa, Dana; Bunning, Bryan J.; Manohar, Monali; Tibshirani, Robert; Fernandez-Becker, Nielsen Q.; Kambham, Neeraja; West, Robert B.
- Abstract
Peanuts in the gut: Peanut allergy affects millions of children and adults worldwide, and peripheral blood IgE+ B cells have been studied in previous peanut allergy studies. Less is known about B cells in tissues exposed to peanuts, and Hoh et al. have now characterized IgE+ B cell clones in the blood, esophagus, stomach, and duodenum in 19 peanut-allergic patients. The stomach and duodenum were enriched for IgE+ B cells with a plasma cell phenotype in allergic patients. High-throughput DNA analysis indicated that tissues were the sites of local isotype switching, and similar antibody sequences for the Ara h 2 peanut allergen were shared between patients. These data better define a role for IgE+ B cells in the gastrointestinal tract in peanut allergy. B cells in human food allergy have been studied predominantly in the blood. Little is known about IgE+ B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE+ clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE+ cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE+ and non-IgE–expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE+ B lineage cells in food allergy.
- Subjects
B cells; PLASMA cells; GASTROINTESTINAL system; FOOD allergy; DNA analysis; IMMUNOGLOBULIN class switching
- Publication
Science Immunology, 2020, Vol 5, Issue 45, p1
- ISSN
2470-9468
- Publication type
Article
- DOI
10.1126/sciimmunol.aay4209