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- Title
Development of CD25.
- Authors
Degauque, Nicolas; Lair, David; Braudeau, Cécile; Haspot, Fabienne; Sébille, Fabien; Dupont, Alexandre; Merieau, Emmanuel; Brouard, Sophie; Soulillou, Jean-Paul
- Abstract
Donor-specific heart allograft acceptance can be induced in the MHC-mismatched LEW.1 W to LEW.1A rat by donor-specific transfusions. Whereas the induction phase of tolerance has been studied in detail, its maintenance remained poorly understood. Here, we performed a side-by-side comparison of CD25 and CD25 splenic T cells of 100-day tolerant rats. Administration of CD25 T cells from tolerant rats to sublethally irradiated recipients transferred long-term graft survival. These CD25 T cells displayed a decreased donor-specific response in the mixed lymphocyte reaction and presented suppressive activity. These CD25 T cells accumulated IFN-γ, IL-10 and Foxp3 transcripts. The in vitro suppressive activity of CD25 T cells required both cell contact and soluble factors (IL-10 and IFN-γ). The CD25 T cells from tolerant rats did not show any modification of their regulatory properties. We show that splenic CD25 T cells of tolerant rats contribute to the maintenance of tolerance following the transplantation. Our data show that regulatory T cells are not restricted to the CD4CD25 T cell subset and provide new insights on the mechanisms of tolerance to allograft following donor cell priming.
- Publication
European Journal of Immunology, 2007, Vol 37, Issue 1, p147
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200635879