We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation.
- Authors
Subramanian, Ayshwarya; Sidhom, Eriene-Heidi; Emani, Maheswarareddy; Vernon, Katherine; Sahakian, Nareh; Zhou, Yiming; Kost-Alimova, Maria; Slyper, Michal; Waldman, Julia; Dionne, Danielle; Nguyen, Lan T.; Weins, Astrid; Marshall, Jamie L.; Rosenblatt-Rosen, Orit; Regev, Aviv; Greka, Anna
- Abstract
Human iPSC-derived kidney organoids have the potential to revolutionize discovery, but assessing their consistency and reproducibility across iPSC lines, and reducing the generation of off-target cells remain an open challenge. Here, we profile four human iPSC lines for a total of 450,118 single cells to show how organoid composition and development are comparable to human fetal and adult kidneys. Although cell classes are largely reproducible across time points, protocols, and replicates, we detect variability in cell proportions between different iPSC lines, largely due to off-target cells. To address this, we analyze organoids transplanted under the mouse kidney capsule and find diminished off-target cells. Our work shows how single cell RNA-seq (scRNA-seq) can score organoids for reproducibility, faithfulness and quality, that kidney organoids derived from different iPSC lines are comparable surrogates for human kidney, and that transplantation enhances their formation by diminishing off-target cells. How reproducible human kidney organoids derived from different iPSC lines are, and how faithful they are to human kidney tissue remain unclear. Here, the authors use four human iPSC lines to derive kidney organoids and show how organoid composition is reproducible, comparable to human tissue and of improved quality after transplantation.
- Subjects
KIDNEY transplantation; RNA sequencing; RNA analysis; ORGANOIDS; SINGLE cell proteins
- Publication
Nature Communications, 2019, Vol 10, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-019-13382-0