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- Title
Landscape of T Cells in NK‐AML(M4/M5) Revealed by Single‐Cell Sequencing.
- Authors
Wu, Wenqi; Liang, Xiaolin; Li, Huiqun; Huang, Xiaoke; Wan, Chengyao; Xie, Qiongni; Liu, Zhenfang
- Abstract
Normal karyotype acute myeloid leukemia (NK‐AML) is a highly heterogeneous malignancy that resides within a complex immune microenvironment, complicating efforts to reveal the interaction between leukemia cells and immune cells. Understanding tumor‐infiltrating T cells is crucial to the advancement of immune therapies and the improvement of the prognosis for NK‐AML patients. We performed single‐cell RNA sequencing on bone marrow cells from 5 NK‐AML (M4/M5) patients and 1 normal donor and paired single‐cell T cell receptor (TCR) sequencing on single T cells. As a result, we identified 8 T cell clusters based on the gene expression characteristics of each subset in NK‐AML and described their developmental trajectories. In NK‐AML patients, specific clusters, such as mucosal‐associated invariant T cells (MAITs), were preferentially enriched and potentially clonally expanded. These transcriptome and TCR data analyses provide valuable insights and rich resources for understanding the immune environment of NK‐AML.
- Subjects
T cells; BONE marrow cells; T cell receptors; ACUTE myeloid leukemia; RNA sequencing
- Publication
Journal of Leukocyte Biology, 2022, Vol 112, Issue 4, p745
- ISSN
0741-5400
- Publication type
Article
- DOI
10.1002/JLB.5A0721-396RR