We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucoside promotes the effects of dental pulp stem cells on rebuilding periodontal tissues in experimental periodontal defects.
- Authors
Lin, Chi‐Yu; Tsai, Min‐Shi; Kuo, Po‐Jan; Chin, Yu‐Tang; Weng, I‐Tsen; Wu, Yen; Huang, Haw‐Ming; Hsiung, Chao‐Nan; Lin, Hung‐Yun; Lee, Sheng‐Yang; Lin, Chi-Yu; Tsai, Min-Shi; Kuo, Po-Jan; Chin, Yu-Tang; Weng, I-Tsen; Huang, Haw-Ming; Hsiung, Chao-Nan; Lin, Hung-Yun; Lee, Sheng-Yang
- Abstract
<bold>Background: </bold>This study aimed to investigate the regenerative effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG)-treated human dental pulp stem cells (DPSC) on the healing of experimental periodontal defects in rats.<bold>Methods: </bold>The maxillary first molars of 30 male Sprague-Dawley rats were extracted, and after healing, bilateral periodontal defects were surgically created mesially in second molars. The defects were treated with Matrigel (as control), DPSC, or DPSC + THSG. After 2 weeks, the healed defects were evaluated using microcomputed tomography and through histological and immunohistochemical analyses.<bold>Results: </bold>In the microcomputed tomography analysis, more new bone formation in the DPSC and DPSC + THSG groups was observed compared with the control group. The periodontal bone supporting ratio in site with DPSC + THSG was significantly higher than that in DPSC. Histologically, an enhanced new bone formation and more significant periodontal attachment were observed in the DPSC + THSG group. The expression levels of proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), and osteopontin (OPN) in the DPSC + THSG group were significantly greater than those in other groups.<bold>Conclusions: </bold>THSG-revolutionized DPSCs significantly shortened the regenerative period of periodontal defects by enhancing the cell recruitment and possibly the angiogenesis in rat models, which illustrate the critical implications for a clinical application and provide a novel tactic for periodontitis treatment.
- Subjects
GLUCOSIDES; DENTAL pulp; STEM cells; PERIODONTAL ligament; WOUND healing; TREATMENT of molar abnormalities; RESEARCH; ANIMAL experimentation; RESEARCH methodology; GLYCOSIDES; MEDICAL cooperation; EVALUATION research; STILBENE; RATS; COMPARATIVE studies; VASCULAR endothelial growth factors; COMPUTED tomography
- Publication
Journal of Periodontology, 2021, Vol 92, Issue 2, p306
- ISSN
0022-3492
- Publication type
journal article
- DOI
10.1002/JPER.19-0563