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- Title
A New Model of Diarrhea with Spleen-Kidney Yang Deficiency Syndrome.
- Authors
Zhu, Jiajie; Liu, Shan; Guo, Yu; Hou, Liwei; Su, Xiaolan; Li, Yijie; Han, Boyu; Liu, Dengke; Wang, Qingguo; Chen, Jiande J. D.; Wei, Wei
- Abstract
Objective. The aim of this study was to examine a new method to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome. Methods. A senna leaf (Folium sennae) decoction was made in 3 concentrations of 1.0, 0.5, and 0.25 g/mL. Rats were randomly divided into 4 groups: the control (C)-, high (H)-, middle (M)-, and low (L)- dose groups. The groups received saline, 1.0, 0.5, or 0.25 g/mL senna leaf decoction, respectively, for 4 weeks. Body weight monitoring, food consumption, water intake, defecation frequency, stool Bristol score, weight-loaded forced swimming test, forelimb grip strength test, D-xylose absorption test, serum cortisone, adrenocorticotropic hormone (ACTH), 24 h urine 17-hydroxycorticosteroid (17-OHCS), and histopathological detection were conducted to assess the success of the senna leaf decoction-induced model. Results. This study showed that the senna leaf decoction could induce diarrhea and dose-dependently slow body weight growth, reduce food consumption, and increase water intake, stool Bristol score, and defecation frequency. Statistical differences were found between groups H and M in rectal temperature, weight-loaded forced swimming time, forelimb grip strength, and serum cortisone. The D-xylose absorption test also showed dysfunction of intestinal absorption in groups H and M. The serum cortisone and 24 h urine 17-OHCS were significantly reduced in group H. Conclusions. Gastric gavage of 10 mL/kg of body weight of a high concentration of a senna leaf decoction (1.0 g/mL) for 4 weeks was used to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome.
- Subjects
ADRENOCORTICOTROPIC hormone; ADRENOCORTICAL hormones; ANIMAL experimentation; BIOLOGICAL models; BODY temperature; BODY weight; CORTISONE; DEFECATION; DIARRHEA; DRINKING (Physiology); GRIP strength; HISTOLOGICAL techniques; INGESTION; INTESTINAL absorption; KIDNEY diseases; LEAVES; CHINESE medicine; MONOSACCHARIDES; RATS; RECTUM; SPLEEN diseases; GASTRIC intubation; SWIMMING; ABSORPTION
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2018, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2018/4280343