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- Title
Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients.
- Authors
Centofanti, Federica; Santoro, Massimo; Marini, Mario; Visconti, Virginia Veronica; Rinaldi, Anna Maria; Celi, Monica; D'Arcangelo, Giovanna; Novelli, Giuseppe; Orlandi, Augusto; Tancredi, Virginia; Tarantino, Umberto; Botta, Annalisa
- Abstract
Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (n = 4), and controls (CTRs, n = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: CEP83-AS1, RP11-84C13.1, CTC-487M23.5, GAS5, NCBP2-AS2, and SDCBP2-AS1. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of CTC-487M23.5, GAS5, and RP11-84C13.1 lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including miR-21-5p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches.
- Subjects
NON-coding RNA; BONES; REGULATOR genes; PATHOLOGY; GENE expression
- Publication
Biomedicines, 2020, Vol 8, Issue 3, p65
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines8030065