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- Title
Use of recombinant epitopes to study the heterogeneous nature of the autoantibodies against thyroid peroxidase in autoimmune thyroid disease.
- Authors
Zanelli, E.; Henry, M.; Malthiery, Y.
- Abstract
Microsomal antigen is often recognized by the sera from patients with autoimmune thyroid disease (AITD). Human thyroid peroxidase (hTPO) is the main component of this antigen. In a previous study, we expressed hTPO cDNA as fusion proteins in prokaryotic vector; we thereby defined seven antigenic peptides by using two rabbit polyclonal anti-hTPO antibodies. In the present study we used the seven epitopes and three widened peptides to define the reactivity pattern of 61 sera from patients with AITD. Thirty-eight of them reacted against al least one of the seven hTPO-restricted epitopes; 14 were negative against the seven determinants but recognized one or two of the extended peptides. Thus, the antibody response against hTPO appeared to be highly heterogeneous in AITD patient sera. Moreover, we demonstrated that the immunodetection of the hTPO on Western blotting with deoxycholate solubilized microsomes can be perfectly correlated with the recognition of one of the epitopes in the region 554-735.
- Subjects
EPITOPES; PROTEINS; METALLOENZYMES; SERUM; BLOOD plasma; PEPTIDES; AUTOANTIBODIES
- Publication
Clinical & Experimental Immunology, 1992, Vol 87, Issue 1, p80
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/1365-2249.ep16067112