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- Title
T lymphocyte activation in systemic lupus erythematosus analysed by proliferative response to nucleoplasmic proteins on nitrocellulose immunoblots.
- Authors
Pham, B. N.; Prin, L.; Gosset, D.; Hatron, P. Y.; Devulder, B.; Capron, A.; Dessaint, J. P.
- Abstract
Polyclonal B cell activity in systemic lupus erythematosus (SLE)may be under T cell control. The use of nitrocellulose irnmunoblots for the analysis of recognition by peripheral blood lymphocytes of nucleoplasmic proteins in SLE patients led to the characterization of significant proliferative responses to 68K (UI RNP): SS-B: B-B' and D (Sm) antigen in 15 of 20 patients. Variations of proliferative response were parallel to disease activity over a follow-up period of ⩾ 6 months. conferring some prognostic value to the assay of lymphocyte response to nucleoplasmic antigens. The pattern of reactivity differs from the corresponding serum antibody profile, and purified T cell suspensions ( >95% pure) were shown to proliferate in response to soluble nucleoplasmic antigens, indicating that T and B cell repertoires against nucleoplasmic proteins may differ. This suggests that activated helper T cells contribute to the fine modulation of B cell reactivity to subcellular particles to determine the particular antibody profile of the patients.
- Subjects
LYMPHOCYTES; SYSTEMIC lupus erythematosus; B cells; ANTIGENS; BLOOD plasma; CELL culture
- Publication
Clinical & Experimental Immunology, 1989, Vol 77, Issue 2, p168
- ISSN
0009-9104
- Publication type
Article